fusionred cd36 c10 plasmid Search Results


fusionred cd36 c10 plasmid  (Addgene inc)
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Addgene inc fusionred cd36 c10 plasmid
Characterization of genes associated with replicative senescence. (A) The cumulative fraction plot of pLI (the probability of being loss-of-function intolerant) value of genes upregulated in old cells (Y<O, orange), genes downregulated in old cells (Y>O, blue) and other protein-coding genes (gray). This measurement is obtained from ExAC database . (B) The cumulative fraction plot of b-value (the index of background selection between primate species) of genes in each category (Mc ). The color scheme is same as the previous panel. (C) X-axis shows the tolerance of genes in healthy human genomes (-log p- value of pLI), y-axis shows the log 2 fold change between young and old in BJ cells and the size of the bubble indicates the log 2 fold change between young and old in MRC-5 cells. The strength of the color shows the -log p-value of pLI. <t>CD36</t> showed upregulation in both old BJ and MRC-5 cells and tolerance to loss-of-function mutations in healthy human genomes.
Fusionred Cd36 C10 Plasmid, supplied by Addgene inc, used in various techniques. Bioz Stars score: 88/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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fusionred cd36 c10 plasmid - by Bioz Stars, 2026-03
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Characterization of genes associated with replicative senescence. (A) The cumulative fraction plot of pLI (the probability of being loss-of-function intolerant) value of genes upregulated in old cells (Y<O, orange), genes downregulated in old cells (Y>O, blue) and other protein-coding genes (gray). This measurement is obtained from ExAC database . (B) The cumulative fraction plot of b-value (the index of background selection between primate species) of genes in each category (Mc ). The color scheme is same as the previous panel. (C) X-axis shows the tolerance of genes in healthy human genomes (-log p- value of pLI), y-axis shows the log 2 fold change between young and old in BJ cells and the size of the bubble indicates the log 2 fold change between young and old in MRC-5 cells. The strength of the color shows the -log p-value of pLI. CD36 showed upregulation in both old BJ and MRC-5 cells and tolerance to loss-of-function mutations in healthy human genomes.

Journal: bioRxiv

Article Title: An evolutionary transcriptomics approach links CD36 to membrane remodeling in replicative senescence

doi: 10.1101/294512

Figure Lengend Snippet: Characterization of genes associated with replicative senescence. (A) The cumulative fraction plot of pLI (the probability of being loss-of-function intolerant) value of genes upregulated in old cells (YO, blue) and other protein-coding genes (gray). This measurement is obtained from ExAC database . (B) The cumulative fraction plot of b-value (the index of background selection between primate species) of genes in each category (Mc ). The color scheme is same as the previous panel. (C) X-axis shows the tolerance of genes in healthy human genomes (-log p- value of pLI), y-axis shows the log 2 fold change between young and old in BJ cells and the size of the bubble indicates the log 2 fold change between young and old in MRC-5 cells. The strength of the color shows the -log p-value of pLI. CD36 showed upregulation in both old BJ and MRC-5 cells and tolerance to loss-of-function mutations in healthy human genomes.

Article Snippet: FusionRed-CD36-C10 plasmid (#56104) was obtained from Addgene (deposited by the Davidson lab).

Techniques: Selection

CD36 overexpression results in senescence-like phenotype. (A) Representative images of control and CD36 -overexpressing young MRC-5 cells. CD36 o verexpression was visualized via the FusionRed tag (scale bar = 10µm). (B-C) Measurement of β-galactosidase activity in control and CD36 overexpressing MRC-5 cells. A significant increase of SA-β-galactosidase positive cells was observed during CD36 overexpression for which representative images are shown (scale bar =100µm for control and center CD36 images, and 20µm for furthest to the right CD36 image). Data shown as means ± standard deviation, *** p < 0.001. (D) Representative western blot showing p16 levels in control and CD36 overexpressing young MRC-5 cells.α-tubulin was used as a loading control. We observe a modest increase in p16 levels. (E) Measurement of β-galactosidase activity in young MRC-5s that were treated with conditioned media. “Control” represents cells in standard growth medium, “Control_M” represents cells in medium from control wells during transfection, “+CD36_M1” represents cells in medium from CD36 overexpressing cells 48h after transfection, and “+CD36_M2” represents cells in medium from CD36 overexpressing cells 96h after transfection. An increase in SA-β-galactosidase positive cells was observed.

Journal: bioRxiv

Article Title: An evolutionary transcriptomics approach links CD36 to membrane remodeling in replicative senescence

doi: 10.1101/294512

Figure Lengend Snippet: CD36 overexpression results in senescence-like phenotype. (A) Representative images of control and CD36 -overexpressing young MRC-5 cells. CD36 o verexpression was visualized via the FusionRed tag (scale bar = 10µm). (B-C) Measurement of β-galactosidase activity in control and CD36 overexpressing MRC-5 cells. A significant increase of SA-β-galactosidase positive cells was observed during CD36 overexpression for which representative images are shown (scale bar =100µm for control and center CD36 images, and 20µm for furthest to the right CD36 image). Data shown as means ± standard deviation, *** p < 0.001. (D) Representative western blot showing p16 levels in control and CD36 overexpressing young MRC-5 cells.α-tubulin was used as a loading control. We observe a modest increase in p16 levels. (E) Measurement of β-galactosidase activity in young MRC-5s that were treated with conditioned media. “Control” represents cells in standard growth medium, “Control_M” represents cells in medium from control wells during transfection, “+CD36_M1” represents cells in medium from CD36 overexpressing cells 48h after transfection, and “+CD36_M2” represents cells in medium from CD36 overexpressing cells 96h after transfection. An increase in SA-β-galactosidase positive cells was observed.

Article Snippet: FusionRed-CD36-C10 plasmid (#56104) was obtained from Addgene (deposited by the Davidson lab).

Techniques: Over Expression, Activity Assay, Standard Deviation, Western Blot, Transfection